A simple procedure is described for the production of large numbers of rooted plantlets from callus cultures of Lotononis bainesii Baker. Highest frequency of shoots was obtained from somatic embryos formed in initiation cultures on Murashige & Skoog medium plus 1 mg/l benzylaminopurine plus 1 mg/l naphthaleneacetic acid. The somatic embryos from these initiation cultures were transferred to media containing 0,5,10 or 20 mg/l isopentenyladenine or 0,1 or 5,0 mg/l kinetin (1,8 to 3,1 shoots per culture) where they re?callused and produced shoots. All shoots obtained from the cultures on media containing 0,5, 10 or 20 mg/l isopentenyladenine or 0,1 mg/l kinetin were rooted; 35 % of shoots from the cultures on medium containing 5,0 mg/l kinetin were rooted. Rooting of unrooted shoots from other treatments was readily induced on medium containing 0,1 mg/l indoleaceticacid.
Journal articles from the Grassland Society of Southern Africa (GSSA) African Journal of Range and Forage Science as well as related articles and reports from throughout the southern African region.