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Embryogenic callus was obtained from immature regions of the leaf of Digitaria eriantha when grown in the dark on a MS medium supplemented with 1,0 or 2,0mg/dm3, 2,4?D and 3?9 % sucrose. Plantlet regeneration occurred when the calli were transferred to MS medium containing 0,1 mg/dm3, 2,4?D and 7?8 % sucrose, and placed under 16 hour photoperiod. The regenerated plantlets were successfully planted out into a mixture of sand and potting soil.