Rangeland Ecology & Management

Get reliable science

Diet sample collection by esophageal fistula and rumen evacuation techniques
Author
Olson, K. C.
Publisher
Society for Range Management
Publication Year
1991-09-01
Body

Two trials were conducted to compare diet samples collected in the evacuated rumen or through the esophageal fistula. Hypotheses tested were (1) rumen evacuation would not decrease selectivity, (2) being in the rumen during collection would not alter the sample, and (3) both techniques accurately estimated nutritional characteristics of the feed offered. Five steers bifistulated at the esophagus and rumen were used in a grazing and a stall trial. Three collection techniques were used in each trial: rumen collection after evacuation (RC), esophageal collection with the rumen evacuated (ECRE), and esophageal collection with the rumen full (ECRF). Comparison of RC and ECRE assessed the influence of being in the rumen, and ECRE vs ECRF tested selectivity. Hay was sampled before feeding in the stall trial to test hypothesis 3. All samples were analyzed for organic matter (OM), nitrogen (N), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), hemicellulose, and cellulose. In the grazing trial, collection technique affected only ADL (P = 0.05), with ECRE depressed compared to ECRF. Organic matter, N, ADL, and hemicellulose responded (P < 0.05) during the stall trial as follows. Salivary ash contamination depressed OM (P = 0.03) in all collected masticate compared to the feed offered. Rumen collection elevated N (P = 0.04), but esophageal samples and feed were equal. Hemicellulose was depressed slightly (P = 0.01) in all collected masticate. Both techniques elevated ADL (P = 0.001), with RC having a greater effect than ECRF. Both collection techniques should provide satisfactory results in grazing trials if precautions are taken. Comparison across techniques appears appropriate if caution is exercised, particularly concerning N and ADL. This material was digitized as part of a cooperative project between the Society for Range Management and the University of Arizona Libraries. The Journal of Range Management archives are made available by the Society for Range Management and the University of Arizona Libraries. Contact lbry-journals@email.arizona.edu for further information. Migrated from OJS platform August 2020

Language
en
Resource Type
Text
Document Type
Journal Issue/Article
Digital Object Identifier (DOI)
10.2307/4002756
Additional Information
Olson, K. C. (1991). Diet sample collection by esophageal fistula and rumen evacuation techniques. Journal of Range Management, 44(5), 515-519.
IISN
0022-409X
OAI Identifier
oai:repository.arizona.edu:10150/644691
Journal Volume
44
Journal Number
5
Journal Pages
515-519
Journal Name
Journal of Range Management
Keywords
rumen
fistulation
grazing trials
rumen contents
chemical composition
sampling
esophagus
cattle
forage
feed intake