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Lepidium sativum is a fast growing annual herb belonging to the Brassicaceae family and possesses the galactogogue effect that promote milk synthesis and production. Study was conducted at the experimental farm of Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.) to evaluate the effect of planting condition and different nutrient sources on soil physicochemica l properties and carbon stock of Morus based Agroforestry system. The study consisted two structural and functional components Morus alba fodder tree as woody perennial and Lepidium sativum as intercrop. There were eight treatments i.e.T1: Lepidium + Morus +FYM@ 4 tonnes ha-1, T 2: Lepidium + Morus +Vermicompost@ 1.12 tonnes ha-1, T 3: Lepidium + Morus +Jeevamrut@ 500Litre ha-1, T 4: Lepidium + Morus +No Manure, T 5: Lepidium + FYM @ 4tonnes ha-1, T 6: Lepidium + Vermicompost @ 1.12 tonnes ha-1, T7: Lepidium + Jeevamrut@ 500Litre ha-1, T8: Control (without Morus and no Manure). Jeevamrut liquid organic manure made up of cow dung, c ow urine, jaggery, gram flour, soil and water. It acts as an agent to increase the microbial count. The present study revealed that the tree proximity and nutrient sources significantly affected the soil physicochemical properties and carbon stock of the system. The results of the study indicated that the highest soil, bulk density (1.35 g cm-3), available Nitrogen (344.60 kg ha-1), Phosphorus (91.76 kg ha-1) Potassium (539.52kg ha-1), organic carbon (10.50 g kg-1), and soil organic carbon stock (21.31 Mg ha-1) was recorded in treatment T 2i.e. Lepidium sativu m+ Morus alba + Vermicompost @ 1.12 tonnes ha-1 while the lowest was recorded in treatment T 8i.e. Control (without Morus and no Manure). Maximum seed yield (1613.07 kg ha-1) was observed in treatment T6 while the lowest was recorded in treatment T4. The treatment T3 (Morus + Lepidium+Jeevamrut) resulted in higher net return (1021.85 USD ha-1) and B:C ratio (2.44) when 500 litres hectare-1 Jeevamrut was applied as organic manure.